김재우 / KIM, Jae-Woo
부교수

Department / Institute / Center :
1. 생화학-분자생물학교실(Dept. Biochemistry and Molecular Biology)
2. 유전과학연구소(Institute of Genetic Science)
3. 만성대사성질환연구센터(Center for Chronic Metabolic Disease Research)
4. 연세대 WCU 융합오믹스 의생명과학과(Yonsei Univ WCU Dept. Integrated OMICS for Biomedical Science)

Contact Information

Phone +82-2-2228-1685 Email japol13@yuhs.ac Website http://biochemistry.yonsei.ac.kr

Educational Background

Undergraduate Education M.D., Yonsei University College of Medicine, 1992
Final Degree (University, Major) Ph.D., Graduate School, Yonsei University, 1998
Overseas Training (Institute, Supervisor) Johns Hopkins University, School of Medicine, Dept. Biological Chemistry
M. Daniel Lane, PhD, 2004-2006

Professional Societies and current positions

생화학분자생물학회(KSBMB), 운영위원, 2009-현재
대한내분비학회, 운영위원, 2007-2010
연세대학교 의과대학 교무부장, 2008-2010
연세의료원 의과학연구처, 연구지원부처장, 2010-현재
BMB Reports, Editor, 2010-현재

Research Area(s)

Basic research, Translational research
Research Field Regulation of adipogenesis
Mechanism and control of hepatic steatosis, diabetes, and obesity
Mesenchymal stem cell differentiation
Transcriptional regulation of glucose/lipid metabolism
Mechanism involved in nuclear receptor-mediated metabolic disease
Diseases DM, obesity, hepatic steatosis, aging, atherosclerosis, metabolic syndrome
Possible Collaboration 지방간 환자에서 간 효소의 변화 및 혈액 진단 마커 개발
지방간 및 간손상을 억제할 수 있는 신약 개발
지방세포 분화를 억제할 수 있는 메커니즘 및 신약 개발
지방세포와 관련된 모든 연구
노화 및 세포 분화와 관련된 연구

Research Methods

Mouse primary hepatocyte isolation and culture
3T3-L1 preadipocyte culture and differentiation
siRNA, shRNA, lentivirus, adenoviral gene transfer (tail vein injection)
Confocal microscopy, immunohistochemistry
FACS analysis (cell cycle)
Chromatin immunoprecipitation, EMSA, transfection and luciferase assay
Recombinant protein preparation
Western blot, RT-PCR, gene cloning
Obesity model mice (diet-induced, ob/ob, db/db)
White adipose tissue and brown adipose tissue
Adipose tissue derived stem cell culture

Lab.

연구실사이트 바로가기
Location of Office 의대신관 501호
Location of Lab. 의대신관 504호
Contact person Name: 김효정(연구교수 / Tel: : 2228-1692 / E-mail: HJKIM17@yuhs.ac

Major Published Articles and Books

Lee H, Lee YJ, Choi H, Ko EH, Kim JW. Reactive oxygen species facilitate adipocyte differentiation by accelerating mitotic clonal expansion. J Biol Chem 284, 10601-10609, 2009

Kim JW, Tang QQ, Li X, Lane MD. Effect of Phosphorylation and S-S Bond-induced Dimerization on DNA Binding and Transcriptional Activation by C/EBP. Proc Natl Acad Sci U S A 104: 1800-1804, 2007

Kim JW, Molina H, Pandey A, Lane MD. Upstream Stimulatory Factors Regulate the C/EBP Gene During Differentiation of 3T3-L1 Preadipocytes. Biochem Biophys Res Commun 354: 517-521, 2007

Park SK, Oh SY, Lee MY, Yoon S, Kim KS, Kim JW. CCAAT/Enhancer Binding Protein and Nuclear Factor-Y Regulate Adiponectin Gene Expression in Adipose Tissue. Diabetes 53: 2757-2766, 2004

Kim JW, Ahn YH. CCAAT/Enhancer Binding Protein Regulates the Promoter Activity of the Rat GLUT2 Glucose Transporter Gene in Liver Cells. Biochem J 336: 83-90, 1998